Services We Provide


Experimentation

  • Validated Ab panels: Mass cytometry provides a powerful way to better understand heterogeneous cell suspensions, like whole blood (WB), peripheral blood mononuclear cells (PBMC), bone marrow mononuclear cells (BMMNCs) or tissue samples. Both mouse and human cells can be analyzed using our validated antibody panels learn more.
  • Sample preparation for Mass Cytometry (CyTOF): Coordinate with the facility contact person before delivering samples to our facility. All the samples should be transported to our facility in a box with Dry ice or Liquid Nitrogen containers. When transporting samples, provide an electronic copy of the sample list as per the instructions here.


Prepping different sample types and protocols

  • PBMCs, BMMNCs and mouse splenocyte sample freezing Cells should be frozen using a cryoprotective media containing 10% dimethyl sulfoxide (DMSO) and 90% fetal bovine serum (FBS) and stored in -80oC or Liquid Nitrogen. Download the protocol here.
  • Human and mouse WB sample preparation WB offers the following advantages when compared with PBMCs
    • WB when stabilized immediately after blood draw is more physiological
    • Less variation with WB vs PBMC prep
    • Unlike PBMCs, WB prevents loss of some immune cell components like granulocytes
    • Technically skilled personnel are needed to isolate PBMCs, while a WB prep can be done at the patient bedside even by a nurse

  • Apply for a Mass Cytometry Service Project

    Researchers from all over Sweden with an interest in understanding the cellular frequencies and functional states in their own samples of interest, are kindly invited to apply for a Mass cytometry service project using our web application form. Please see the introduction here or enter from the Project Portal.